Vitellogenin Assay by Enzyme-Linked Immunosorbant Assay as a Biomarker of Endocrine Disruptor Chemicals Pollution
نویسندگان
چکیده
منابع مشابه
Vitellogenin assay by enzyme-linked immunosorbant assay as a biomarker of endocrine disruptor chemicals pollution.
Research is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals and in the focus of this development are the fish test species common carp (Cyprinus carpio). In this study we have developed quantitative enzyme linked immunosorbent assays (ELISA) for VTS in common carp. The working range of the ELISA was 11.25 to 2000 ng mL(-1) (75-25% specific b...
متن کاملVitellogenin assay by enzyme-linked immunosorbent assay (ELISA) as a biomarker of endocrine disruptor chemicals (EDCs) pollution
There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...
متن کاملvitellogenin assay by enzyme-linked immunosorbent assay (elisa) as a biomarker of endocrine disruptor chemicals (edcs) pollution
there are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals edcs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. the yolk protein precursor vitellogenin (vtg) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to edcs especially...
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A rapid, sensitive, specific and high through-put enzyme-linked immunosorbant assay (ELISA) methodfor determination of morphine in urine samples using penicillinase as label enzyme has been developed. Noextraction or chromatography was included in this assay procedure. Immunoglobulin (Ig) purified polyclonalanti-bodies against a C6-hemisuccinate derivative of morphine (M-C6-HS...
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A method is described by which complement fixation is detected with an enzyme linked immunosorbant assay (ELISA) technique. The method obviates the need for sensitised sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2007
ISSN: 1028-8880
DOI: 10.3923/pjbs.2007.3109.3114